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  • Mbrown02
    Junior Member
    • Mar 2024
    • 2

    #1

    Next Seq 2K Formamide Disposal Question

    Hi, we are in the works of determining how to properly dispose of the formamide from P3 100 Cycle Kits in accordance with Laws and Regulations. Does anyone know if you HAVE to do the optional purge to get all of the formamide out of the Kits, and into the waste? Additionally, would anyone know which location on the picture below is the formamide location? Most other Illumina systems make disposing of the formamide much more seemless, and from reading FAQs, Reference Materials, and Product Documentation it is not the clearest. Thank you!
  • SnapSeq
    Junior Member
    • Apr 2024
    • 8

    #2
    The NextSeq 2000 formamide waste isn't accessible to the general user, what you would need to do is instruct the instrument to do a reagent purge (this dumps any excess volume of all reagents into the common waste). Instructions for how to do this are posted by Illumina here: https://knowledge.illumina.com/instr...list/000003039

    Then, you'll need to empty that waste from the spent reagents compartment by removing the rubber plug at the end of the cartridge. The % formamide is shared by Illumina here: https://knowledge.illumina.com/instr...list/000001544

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