Which approach gives a better NGS library yield and consistency:
- Performing a clean‑up on the entire PCR product (e.g. all 45 µL) and then diluting the eluted DNA to 0.2 ng/µL,
or - Taking 10 µL of the PCR product, adding 40 µL of nuclease‑free water, performing the clean‑up on this diluted sample, and then diluting the eluted DNA to 0.2 ng/µL?**