I have a few total RNA samples that I would like to prepare for the Illumina GA platform, however they are just below the threshold stated in the protocol for processing for mRNA-seq. They recommend 1-10ug total RNA, but I have 800ng in my sample. Has anyone validated this protocol for lower amount of starting material (total RNA)? I would like to avoid pre-amplification of my RNA if possible. Cheers!
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM