[Geneus]

It also suggests massive potential for improved performance over time (by increasing cluster density).

Maybe more "headspace" by driving scanning/optics?

[M4TTN]

Perhaps they are using slower flows to use relatively less reagents. Patent linked in #18.


This is analogous to "Intel's" business strategy. Release just enough "innovation" to remain a comfortable step ahead of competitors.

Interesting idea.

Indeed, the relative lack of competition in both CPUs and NGS is a concern for those doing research on a limited budget.

I'd like to see someone reverse engineer a flowcell and compete on reagent costs, but I doubt it will happen.

[GenoMax]

Does fatter lanes = more reagents = more expensive per run?

Perhaps they are using slower flows to use relatively less reagents. Patent linked in #18.

It also suggests massive potential for improved performance over time (by increasing cluster density).

This is analogous to "Intel's" business strategy. Release just enough "innovation" to remain a comfortable step ahead of competitors.

[GenoMax]

I hope illumina will reconsider "10 to a pack" decision for X in future (there can't be more than a handful customers at that level and 3 are already on board).

More than a handful places can use one (or two) X's.

[dfhdfh]

The rep at PAG said they planned on selling 4 "X boxes" in 2014--and had already sold 3 30 minutes after the announcement.

That is insane.

[M4TTN]

See flowcell pics on Twitter @nextgenseek
Fat lanes = different optics
Probably not a typo

Does fatter lanes = more reagents = more expensive per run?

It also suggests massive potential for improved performance over time (by increasing cluster density).

We just received a refurb MiSeq in December 2013, so I'm somewhat dismayed by the NextSeq500 release. However 250k is well out of budget.

One wonders if the MiSeq will still get improvements. I notice from the HiSeqX spec sheet that they are now using ordered arrays. I wonder how much of an improvement this could yield on the MiSeq, which already has a very high cluster density.

The two things I'd really like to see is MiSeq hit 100M clusters and the cost of reagents per run to halve.

[BBoy]

Interested to see that the cluster density specs are an order of magnitude lower on the 500 than the MiSeq. Is that an error?

Quote from Illumina:
(http://www.illumina.com/systems/next...fications.ilmn)
* Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.

See flowcell pics on Twitter @nextgenseek
Fat lanes = different optics
Probably not a typo

[AllSeq]

Called it! Called it!

Holy sh_!

"NextSeq"

I think you mean "Nailed it!" - really good call!

[SNPsaurus]

New patent on the exclusion amplification for the X. http://bit.ly/1j6sSll

Just transport the dna fragments slowly and amplify quickly.

[ECO]

What I'd like is some more detailed explanation of the 2-dye system the NextSeq 500 is using. I assume they provide C & A tagged with Red, T & A tagged with green & G untagged, thus C should be pure Red, T pure green and A ~ a 50:50 mix of green & red (orange) and G then undyed...

Spec Sheet from ILMN:
http://bit.ly/1iOYN7e

[bilyl]

Called it! Called it!

Holy sh_!

"NextSeq"

Looks like I'll have to eat my hat! Exciting news!

[Richard Finney]

Illumina announces $1,000 genome

??

http://www.utsandiego.com/news/2014/jan/14/illumina-thousand-dollar-genome/

"The cost of sequencing a human genome has at last been brought below $1,000, DNA sequencing giant Illumina said Tuesday. Crossing that barrier opens to door to bringing the public the full benefit of 21st-century genomic medicine."

via hacker news ... https://news.ycombinator.com/item?id=7060021

[M4TTN]

Interested to see that the cluster density specs are an order of magnitude lower on the 500 than the MiSeq. Is that an error?

Quote from Illumina:
(http://www.illumina.com/systems/next...fications.ilmn)
* Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.

[aeonsim]

What I'd like is some more detailed explanation of the 2-dye system the NextSeq 500 is using. I assume they provide C & A tagged with Red, T & A tagged with green & G untagged, thus C should be pure Red, T pure green and A ~ a 50:50 mix of green & red (orange) and G then undyed...

[SNPsaurus]

The rep at PAG said they planned on selling 4 "X boxes" in 2014--and had already sold 3 30 minutes after the announcement.

The NextSeq500 is clearly aimed at people considering a Proton. Same price, same emphasis on speed, better stats right now. I don't like that the only single-end mode is 75 bp. Lots of people like the 150 bp read on the 2500 Rapid. And the other downside is that all 4 lanes (100M reads each) get fed the same library, so doesn't improve flexibility in flow of projects at a facility. But a nice mid-machine.