Dear Guys
I'm starting a metagenomic analysis on the rats fecal microbial community.
But our lab doesn't have any experience about this work.
We will have 96 rats fecal samples and plan to run 16s rRNA gene sequencing by Illumina Miseq.
I still confused about some question after reading papers and protocols.
1.About the depth. How much data is enough for per sample? Some one only got 3M/per sample.
2.How to choose the right reference database for data analysis?
Any help would be greatly appreciated!
I'm starting a metagenomic analysis on the rats fecal microbial community.
But our lab doesn't have any experience about this work.
We will have 96 rats fecal samples and plan to run 16s rRNA gene sequencing by Illumina Miseq.
I still confused about some question after reading papers and protocols.
1.About the depth. How much data is enough for per sample? Some one only got 3M/per sample.
2.How to choose the right reference database for data analysis?
Any help would be greatly appreciated!
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