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  • Chip-Seq libraries

    Hi,
    I'm doing a Chip-Seq experiment (preparing the libraries) and, for some of the samples, the Bioanalyzer peak trace in the final library validation is very very low.
    I suppose I have enought material to continue but it will be very difficult to quantify these samples.
    Is someone experienced with this problem (very low peaks)? Is it frequent?
    Thanks

  • #2
    hi,
    my situation is even worse than you. I can not detect any DNA in Bioanalyzer after ChIP-seq library construction.

    Could anybody help us to figure our what is wrong?
    thanks a lot!

    Comment


    • #3
      How did you measure your starting material concentration?
      Didi you notice any leak on your gel (size selection step)?

      Comment


      • #4
        hi, I only measured once of my ChIP-enriched DNA by Picogreen. It is around 100ng.

        I did not check the leak in my gel. It is a good point. Next time I should do it.

        Thanks
        Shilh

        Comment

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