Hi all!
I am new to NGS technology and I am trying to understand all the steps involved in the protocol going from seq library preparation to the actual NGS run on Hiseq instrument. During library prep, two different adapters are ligated at the DNA fragments. As far as I understand, this is needed for the bridge amplification later on in the flow cell, since this surface is covered with the complements of the two adapters. What I do not understand is why two different adapters are needed and not only one is sufficient? Maybe this is a very naive question and I am overlooking something very basic... but I don't get it.
I made a simplified drawing to illustrate my question (see attach).
Thanks!
I am new to NGS technology and I am trying to understand all the steps involved in the protocol going from seq library preparation to the actual NGS run on Hiseq instrument. During library prep, two different adapters are ligated at the DNA fragments. As far as I understand, this is needed for the bridge amplification later on in the flow cell, since this surface is covered with the complements of the two adapters. What I do not understand is why two different adapters are needed and not only one is sufficient? Maybe this is a very naive question and I am overlooking something very basic... but I don't get it.
I made a simplified drawing to illustrate my question (see attach).
Thanks!
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