Hi,
I have got an illumina DNA genome re-sequencing data. All the items in FastQC reports are satisfactory but "Per sequence GC content". There is a minor peak close to the main peak (please see the attached fig).
All the adapter sequences and low quality reads have already been removed, so I don't think the extra peak is caused by these sequences.
I would appreciate it if you have got some idea what is the reason of the funny shape of the peak and what I should do to correct it.
Thanks in advance!
I have got an illumina DNA genome re-sequencing data. All the items in FastQC reports are satisfactory but "Per sequence GC content". There is a minor peak close to the main peak (please see the attached fig).
All the adapter sequences and low quality reads have already been removed, so I don't think the extra peak is caused by these sequences.
I would appreciate it if you have got some idea what is the reason of the funny shape of the peak and what I should do to correct it.
Thanks in advance!
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