NuGEN
If you believe the problem is with the Illumina adapters or kits themselves perhaps you should try the library prep kits from NuGEN. We have been using them with good success for the past 6 months. They are compatible with all versions of flowcells and chemistry and offer 384 barcodes now. The barcodes are the first thing you sequence so there is also no need for the third index read. The NuGEN adapters are also compatible with the KAPA quantitation kit. Good luck.
If you believe the problem is with the Illumina adapters or kits themselves perhaps you should try the library prep kits from NuGEN. We have been using them with good success for the past 6 months. They are compatible with all versions of flowcells and chemistry and offer 384 barcodes now. The barcodes are the first thing you sequence so there is also no need for the third index read. The NuGEN adapters are also compatible with the KAPA quantitation kit. Good luck.
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