I am trying to simulate Illumina paired end reads for DNASeq. I need to introduce sequencing errors( substitutions as of now). In latest Illumina sequencing, is there any bias in the substitution? eg. Can a base 'A' change to anything else( C,G or T) with equal probability or are their any specific substitutions which are more likely to occur?
Found an article - journal.embnet.org/index.php/embnetjournal/article/viewFile/667/860
Any other references?
Found an article - journal.embnet.org/index.php/embnetjournal/article/viewFile/667/860
Any other references?