Hi everyone,
I am planning to do RNA-Seq on RNA extracted from breast cancer cells, the main purpose is to identify differentially expressed genes after stimulation. Now I got two quotations from two companies.
Company A: single end 50bp, 10M clean reads per sample
Company B: paired end 2x100bp, 10M reads per sample
The price from company B is 25% lower than company A. This is quite confusing, isn't PE and longer reads more expensive?
Can anyone give me some suggestions? That will help me a lot because I am really new to the sequencing technology..Thanks a lot!
Best,
Frank
I am planning to do RNA-Seq on RNA extracted from breast cancer cells, the main purpose is to identify differentially expressed genes after stimulation. Now I got two quotations from two companies.
Company A: single end 50bp, 10M clean reads per sample
Company B: paired end 2x100bp, 10M reads per sample
The price from company B is 25% lower than company A. This is quite confusing, isn't PE and longer reads more expensive?
Can anyone give me some suggestions? That will help me a lot because I am really new to the sequencing technology..Thanks a lot!
Best,
Frank
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