This question really boils down to the following: If you were to do an RNA-seq experiment today (let's limit the sequencing to illumina) what protocols/kits would you use?
*For single-cell / low-volume (let's assume a few hundred cells)
*For a typical bulk treatment/control experiment
As a bit of background, I've got a few ideas right now that involve
*Demonstrating biases in polyA-selection
*Quantitatively assessing the single-cell seq process
I am wondering whether the first will be of negligible impact because tagmentation makes polyA selection less common, and that left me wondering about the single-cells. Are there clear frontrunners in these areas of prep?
*For single-cell / low-volume (let's assume a few hundred cells)
*For a typical bulk treatment/control experiment
As a bit of background, I've got a few ideas right now that involve
*Demonstrating biases in polyA-selection
*Quantitatively assessing the single-cell seq process
I am wondering whether the first will be of negligible impact because tagmentation makes polyA selection less common, and that left me wondering about the single-cells. Are there clear frontrunners in these areas of prep?
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