I'm using NEB Ultra kit after sonication.
If I run part of sample after adaptor ligation and compare to input DNA, will I be able to see some length change of DNA smear on Egel?
The difference is about 60 bp if ligated.
I'm trying to see whether the ligation works or not because I recently have very low PCR efficiency in the last step. The kit is new so I don't think it's the problem of PCR mix.
I'm wondering if that is because the ligation step didn't work well.
If I run part of sample after adaptor ligation and compare to input DNA, will I be able to see some length change of DNA smear on Egel?
The difference is about 60 bp if ligated.
I'm trying to see whether the ligation works or not because I recently have very low PCR efficiency in the last step. The kit is new so I don't think it's the problem of PCR mix.
I'm wondering if that is because the ligation step didn't work well.
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