We are half-way through our first MiSeq run (NexteraXT v3 2x300bp). Sample is 16 plex of genomic DNA.
Some questions about the BaseSpace reporter:
1. Is it normal for the "intensity" to increase with cycle number? (from 400 to 800 for C and T bases)
2. Is it normal for the % base composition to drift over the run? (from 20% C to 28% C; yet from 20% G to 16% G).
What could cause the latter?
Median Q-score seems OK (I think!).
I've also copied the run summary so far for further info (cluster density = 1400, 1.5% mapped PhiX, etc)
Here are some screen grabs.
Cheers!
Some questions about the BaseSpace reporter:
1. Is it normal for the "intensity" to increase with cycle number? (from 400 to 800 for C and T bases)
2. Is it normal for the % base composition to drift over the run? (from 20% C to 28% C; yet from 20% G to 16% G).
What could cause the latter?
Median Q-score seems OK (I think!).
I've also copied the run summary so far for further info (cluster density = 1400, 1.5% mapped PhiX, etc)
Here are some screen grabs.
Cheers!
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