We are planning to sequence a large number (>10) of genomes. All are approximately 40Mb in size.
We are planning to sequence them on the Illumina Hiseq platform.
Obviously we would like to get the best assemblies possible from the data, however we do not have the funds for Mate-Pair sequencing for all of the genomes.
We are considering sequencing 2 Pair-end libraries of different insert sizes (e.g. 300kb and 600kb) for these genomes.
Has anyone done this before? And would you say that the cost of doing a second PE library was worth the improvement to the genome assembly?
We are planning to sequence them on the Illumina Hiseq platform.
Obviously we would like to get the best assemblies possible from the data, however we do not have the funds for Mate-Pair sequencing for all of the genomes.
We are considering sequencing 2 Pair-end libraries of different insert sizes (e.g. 300kb and 600kb) for these genomes.
Has anyone done this before? And would you say that the cost of doing a second PE library was worth the improvement to the genome assembly?
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