Hello All,
I have a customer with their barcode at the very beginning of their read 1. I consistently am seeing a low number of raw reads as well as those PF. I am told it is a base diversity issue causing the HiSeq to not call all the clusters but I am not sold but am not certain how spot on this needs to be (Base diversity below). Any advice or insight would be greatly appreciated!
Thanks very much!
Cycle 1 2 3 4 5 6
A 25% 29% 38% 29% 29% 29%
T 42% 17% 29% 21% 21% 29%
C 13% 29% 17% 33% 17% 25%
G 21% 25% 17% 17% 33% 17%
I have a customer with their barcode at the very beginning of their read 1. I consistently am seeing a low number of raw reads as well as those PF. I am told it is a base diversity issue causing the HiSeq to not call all the clusters but I am not sold but am not certain how spot on this needs to be (Base diversity below). Any advice or insight would be greatly appreciated!
Thanks very much!
Cycle 1 2 3 4 5 6
A 25% 29% 38% 29% 29% 29%
T 42% 17% 29% 21% 21% 29%
C 13% 29% 17% 33% 17% 25%
G 21% 25% 17% 17% 33% 17%
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