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Originally posted by Brian Bushnell View PostExcept that I'm pretty sure the X-series do use 2-dye chemistry, the same as NextSeq...
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This http://blog.illumina.com/blog/illumi...eq-4000-system says the 4000 uses 4-color.
This http://www.illumina.com/content/dam/...0-2014-057.pdf says that 1 flowcell does 50 transcriptomes per run at 50M reads each, so that is >300M reads per lane. Looks faster as well, if the longest run time is 3.5 days (not sure if that would be for 1x150bp or 2x150bp).Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Heard from someone that the cluster and reagent kits are similar in price to current Hiseq 2000 reagents, which would mean the price per read is quite a bit lower.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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#SNPsaurus:
I assume it will be 2x 150 bp in 3.5 days.
2.1 to 2.5 billion clusters per flow cell, in a 2x150 bp run is 630 - 750 Gb.
With a speed >200Gb per day a 2x 150 bp run will take 3.5 days.
I wonder if we can upgrade the 2500 V4 machine to a 4000 version...
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Originally posted by GenoMax View PostPossible upgrade path for current 2500 owners?
I am not going to hold my breath though.
Seems like they are following the Apple product release model...
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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