Our institute is having trouble completing these long runs. They seem to die towards the end of both reads, camera goes out of focus, etc. They are being run on an upgraded HS2000. FYI, the samples all passed on MiSeq 2x300. We are looking for slightly more yield meta, amp.Has anyone had similar issues, any success, any advice?
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I guess it depends on how good of success you're after. We had a 2x250 v2 rapid run finish this morning and the results were relatively good. There was decreased quality toward the end of each read, but this was due primarily to two tiles (I think they were the exact same ones for each of the reads), so it turned out that the vast majority of reads were quite good. I should note that there was a bigger issue with the end of read 2, where a large block of tiles had problems. The latter issue combined with the same tiles showing some quality problems in both read 1 and 2 would be compatible with a camera problem, since there was an obvious per-tile difference...thereby suggesting that the problem isn't due to the chemistry itself.
I can't give any advice, as I don't personally deal with the sequencer, but that's at least the sort of thing that we're getting at the moment.
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