Hello All,
I am planning to use a Nextera XT library prep kit (FC-131-1024) for my current project. The lab I work in already uses the other Nextera Kit (FC-121-1030) and therefore has the indices for that (non-XT) kit (FC-121-1011).
Does anyone know if the non-XT indices work for the XT kit? Illumina Technical Support said it isn't recommended, but they have heard of individuals "accidentally" using indices for the other kit and it still working. They couldn't recommended this option, but it "might" work.
I asked if the two kits (FC-121-1011 and FC-131-1001) were different and, of course, that information is proprietary.
Any help would be appreciated,
Angus
I am planning to use a Nextera XT library prep kit (FC-131-1024) for my current project. The lab I work in already uses the other Nextera Kit (FC-121-1030) and therefore has the indices for that (non-XT) kit (FC-121-1011).
Does anyone know if the non-XT indices work for the XT kit? Illumina Technical Support said it isn't recommended, but they have heard of individuals "accidentally" using indices for the other kit and it still working. They couldn't recommended this option, but it "might" work.
I asked if the two kits (FC-121-1011 and FC-131-1001) were different and, of course, that information is proprietary.
Any help would be appreciated,
Angus
Comment