Hello everyone,
I have come across instances or articles stating that the reads with quality as only 'BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB' are meant to be LOW quality as they are called read-quality indicators. They define that it is the 3' end of the read which must be removed or filtered to prevent mis-assembly.
Is this true or is that okay to ignore?
Also, If i want to quality filter my illumina sequence file then is there any available tool or any speicific feature to recognise bad quality reads? so that I can create a custom program to do so if not ready..
I know this might be a trivial question, but I am new to Illumina technology. Kindly help.
Thanks in advance!
I have come across instances or articles stating that the reads with quality as only 'BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB' are meant to be LOW quality as they are called read-quality indicators. They define that it is the 3' end of the read which must be removed or filtered to prevent mis-assembly.
Is this true or is that okay to ignore?
Also, If i want to quality filter my illumina sequence file then is there any available tool or any speicific feature to recognise bad quality reads? so that I can create a custom program to do so if not ready..
I know this might be a trivial question, but I am new to Illumina technology. Kindly help.
Thanks in advance!
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