Hi,
I am newbie. I am working on de novo assembly of human genome.
I am new in the field, but not getting any help. So I would appreciate any sort of help to proceed.
I made the DNA library, and I got my sequencing data from the sequencing facility. They ran my samples on Illumina MiSeq 2x250. This is a 250 bp PE MiSeq multiplex run. I have 4 samples, paired end reads, each has R1 and R2.
I checked the quality by running on FastQC. I chose to run SOAP de novo I downloaded SOAP de novo assembler file : SOAPdenovo2-src-r240.tgz in my Linux, but I am not sure how to start.
I unzip the file by the command:
tar -zxf /home/mydir/SOAPdenovo2-src-r240.tgz
Then, checked by
ls -l
and saw SOAPdenovo2-src-r240 file
Next, I changed directory to SOAPdenovo2-src-r240 by
cd SOAPdenovo2-src-r240
and saw all its contents.
Now, what would be the command to load my data file? What is the configuration file, and how do I load that?
Please help.
Thanks,
I am newbie. I am working on de novo assembly of human genome.
I am new in the field, but not getting any help. So I would appreciate any sort of help to proceed.
I made the DNA library, and I got my sequencing data from the sequencing facility. They ran my samples on Illumina MiSeq 2x250. This is a 250 bp PE MiSeq multiplex run. I have 4 samples, paired end reads, each has R1 and R2.
I checked the quality by running on FastQC. I chose to run SOAP de novo I downloaded SOAP de novo assembler file : SOAPdenovo2-src-r240.tgz in my Linux, but I am not sure how to start.
I unzip the file by the command:
tar -zxf /home/mydir/SOAPdenovo2-src-r240.tgz
Then, checked by
ls -l
and saw SOAPdenovo2-src-r240 file
Next, I changed directory to SOAPdenovo2-src-r240 by
cd SOAPdenovo2-src-r240
and saw all its contents.
Now, what would be the command to load my data file? What is the configuration file, and how do I load that?
Please help.
Thanks,