Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Increase the number of cycle with a 50 cycles MiSeq Reagent Kit v2

    Hello,

    Is it possible to do a run of 60 cycles with a 50 cycles MiSeq Reagent Kit v2 ?

  • #2
    Hi jsvinco,
    Each kit is loaded with 25 additional cycles for indexing. So unless you need both indexing reads the answer is a 'yes'. If you need both indexing reads as well as the 50 cycles you might be pushing it - we have pushed the machine 5 cycles over the total cycle count (i.e 330 cycles on a 300 kit) and it behaved (although the machine complains at set-up). Other might have gone further?

    Hope this helps - Cheers, Mike.

    Comment


    • #3
      Thank you for your help. It's very helpful to now that.

      jsvinco

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Quality Control Essentials for Next-Generation Sequencing Workflows
        by seqadmin




        Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.

        Nucleic Acid Quality Control
        Preparing for NGS starts with isolating the...
        02-10-2025, 01:58 PM
      • seqadmin
        An Introduction to the Technologies Transforming Precision Medicine
        by seqadmin


        In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...
        01-27-2025, 07:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 02-07-2025, 09:30 AM
      0 responses
      72 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-05-2025, 10:34 AM
      0 responses
      113 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-03-2025, 09:07 AM
      0 responses
      87 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 01-31-2025, 08:31 AM
      0 responses
      48 views
      0 likes
      Last Post seqadmin  
      Working...
      X