By how much 5' ends of custom sequencing primers are allowed to overlap with the P5 and P7 flow cell adapters on MiSeq cell?
More specifically, with these sequences:
P5: 5' AATGATACGGCGACCACCGA 3'
P7: 5' CAAGCAGAAGACGGCATACGA 3'
Has anyone tried complete overlap?
Thanks
More specifically, with these sequences:
P5: 5' AATGATACGGCGACCACCGA 3'
P7: 5' CAAGCAGAAGACGGCATACGA 3'
Has anyone tried complete overlap?
Thanks
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