$550 is for catalog # FC-420-1004, which is a "mid output" 300 cycle kit. So up to 8 million reads. High output kits for 300 cycles and all 25M reads costs $1500 (FC-420-1003), also not counting sample prep. Costly little fella for OpEx.
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Originally posted by misterc View Post$550 is for catalog # FC-420-1004, which is a "mid output" 300 cycle kit. So up to 8 million reads. High output kits for 300 cycles and all 25M reads costs $1500 (FC-420-1003), also not counting sample prep. Costly little fella for OpEx.
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300 cycles and 16M reads (Illumina spec) for a MiSeq v2 kit is $1000. So, you're getting more MiniSeq reads but for a price increase that ends up keeping the cost per base about... yeah, the same. (~2E-5 cents per base if my napkin math is right)
And what JBKri said about error rates-- comparing to one of my last MiSeq runs, the error rates are about double. Is that typical for the 2-dye chemistry?
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Okay, so basically no cost-per-read advantage over the MiSeq either. I don't mind paying an extra $50 per run to double my read lengths. But if I were buying a new machine, that wouldn't be worth the extra $100k down payment plus the maintenance, so there's a place for it.
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Originally posted by Jessica_L View PostAnd what JBKri said about error rates-- comparing to one of my last MiSeq runs, the error rates are about double. Is that typical for the 2-dye chemistry?
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2-channel imaging will always struggle to keep the intensities straight amongst the 3 lit bases in 2 images. Everything gets dimmer and less pure in signal as you go from cycle to cycle. This likely is what will drive higher error rates, as I doubt the SBS chemistry is to blame.
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Originally posted by jwfoley View PostOkay, so basically no cost-per-read advantage over the MiSeq either.
Overally though, looks like it is targeted to researchers seeking to trade off the lower up front cost for slightly higher run costs and reduced capacity.Last edited by Ingeneious; 01-26-2016, 02:43 PM.
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Info from Illumina:
The MiniSeq is similar to the technology utilised with our NextSeq platform based on 2-channel SBS chemistry.
Pricing Information
MiniSeq System (SY-420-1001): £35,653
MiniSeq Basic Plan (20004132): £3,350 for 1 additional year’s cover
MiniSeq Comprehensive Plan (20004133): £4,021 for 1 additional year’s cover
Code:High Output Kit 2 x 150 (300bp) 7.5 25 FC-420-1003 1,046 High Output Kit 2 x 75 (150 bp) 3.75 25 FC-420-1002 652 High Output Kit 1 x 75 (75 bp) 1.875 25 FC-420-1001 558 Mid Output Kit 2 x 150 (300 bp) 2.4 8 FC-420-1004 384
Output for counting experiments is the same as MiSeq v3 chemistry (25 M reads)
The only thing it lacks are long paired reads 2x300 bp (but those kits are failing at the moment anyway...) and with NexteraXT many of our fragments are shorter than 300 bp anyway...
I think knowing what I know now...I'd probably buy one...specs are only going to improve.
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