Paritial P7 on MiSeq/HiSeq
Hello,
I have a custom design DNA libraries (~140bp amplicons) that have full-length P5 + SP1 (very same to typical Illumina libraries) but a partial P7 (missing the last "GAT"; CAA GCA GAA GAC GGC ATA CGA).
Does anyone know whether the partial P7 sequence is problematic on MiSeq?
Although I do not know why, I heard that MiSeq and HiSeq-PE require full-length P7 sequence, whereas HiSeq-SR does not.
The library design has been used successfully by other groups on HiSeq-SR, but when I tried on MiSeq, I had a very low clustering density and many of the retrieved reads were quite junk (only a very few reads had the expected sequences).
Any thoughts are appreciated!
I have a custom design DNA libraries (~140bp amplicons) that have full-length P5 + SP1 (very same to typical Illumina libraries) but a partial P7 (missing the last "GAT"; CAA GCA GAA GAC GGC ATA CGA).
Does anyone know whether the partial P7 sequence is problematic on MiSeq?
Although I do not know why, I heard that MiSeq and HiSeq-PE require full-length P7 sequence, whereas HiSeq-SR does not.
The library design has been used successfully by other groups on HiSeq-SR, but when I tried on MiSeq, I had a very low clustering density and many of the retrieved reads were quite junk (only a very few reads had the expected sequences).
Any thoughts are appreciated!