I will be doing MiSeq run for 16S metagenomics. it will be my first time. Recently some MiSeq v3 300 PE runs gave me trouble and hence I am worried. Are there any things I should do differently from what the 16S protocol says. Illumina asks to spike at 25%, should it be that high.
What other things should be taken into consideration while preparing the libraries. I will have at least 96 samples. Can I pool all on PE 300 v3. Please advise and provide feedback/suggestions, anything and everything there can be for this kind of run.
Thanks in advance
What other things should be taken into consideration while preparing the libraries. I will have at least 96 samples. Can I pool all on PE 300 v3. Please advise and provide feedback/suggestions, anything and everything there can be for this kind of run.
Thanks in advance
Comment