I am having trouble determining why certain hypervariable regions are targeted. The papers I have reviewed all seem to say it depends on your study, such as whether you're interested in the rare biosphere or specific types of bacteria or archaea.
However, I am not necessarily sure what should be used for environmental samples from the deep ocean, which is what I am looking at in my project. I will also be using Illumina MiSeq. Some papers I have looked at use V1-V2 and others use V6 or V6-V8, however a reason isn't provided.
Any advice would be greatly appreciated!
However, I am not necessarily sure what should be used for environmental samples from the deep ocean, which is what I am looking at in my project. I will also be using Illumina MiSeq. Some papers I have looked at use V1-V2 and others use V6 or V6-V8, however a reason isn't provided.
Any advice would be greatly appreciated!
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