So my protocol is using a custom P5 index adapter with a standard Illumina Nextera N70X oligo. I made the mistake of using a P5 aliquot that is somewhere between 10 and 100x more concentrated than it should have been.
Aside from primer dimer formation which I can fix, are there any other potential issues this may have caused?
I need to decide whether I should toss these libraries and run tagmentation on a new batch, or send this batch to sequencing after PCR purification.
Aside from primer dimer formation which I can fix, are there any other potential issues this may have caused?
I need to decide whether I should toss these libraries and run tagmentation on a new batch, or send this batch to sequencing after PCR purification.
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