We sent a custom read 1 seq primer that works fine on our NextSeq and MiSeq to two groups with MiniSeqs, and the result is a total failure to find clusters for both. Control runs (Phi X and priming with standard seq primer) confirm the libraries and instruments are working fine, and Illumina TS says annealing temperatures are the same on NextSeq and MiniSeq.
What else could be going wrong here? Has anyone successfully used a custom primer on a MiniSeq?
What else could be going wrong here? Has anyone successfully used a custom primer on a MiniSeq?
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