Paired-end sequencing with barcodes

[Seq_learner]

Hi everyone,

I want to use Miseq for PCR amplicon library. I would like to ask if it is possible to sequence a paired-end read with barcodes like the following structure:

[P5 tail][Seq primer 1][NNNN][Target primer Forward][Target][Target primer Reverse][NNNN][Seq primer 2][P7 tail]

Regards

seq-learner

[jdk787]

That looks fine. Both your R1 and R2 will start with NNNN sequences.

[Seq_learner]

Thank you for your reply