Hi all,
We did a Miseq run (read 1-48 cycles, read 2-50 cycles) on a custom made library. The read quality for read 1 is OK but the read 2 quality is very low. Since it is an amplicon library, the client added some stuffer nucleotides for color balancing.
I attached some SAV screen captures since I don't have the FastQC data yet. Should we suspect an issue with the library construction or could it be faulty reagents?
Thank you very much for your help.
We did a Miseq run (read 1-48 cycles, read 2-50 cycles) on a custom made library. The read quality for read 1 is OK but the read 2 quality is very low. Since it is an amplicon library, the client added some stuffer nucleotides for color balancing.
I attached some SAV screen captures since I don't have the FastQC data yet. Should we suspect an issue with the library construction or could it be faulty reagents?
Thank you very much for your help.
Comment