Hello,
I got some illumina data which obviously has very small insert size as many of the read pairs have >95% overlap. I went back to illumina library protocol and feel that it generally suggests a library insert size longer than the total length of the paired-end read, that is, read pair does not overlap.
What is the optimal library insert size? Should I move forward for data analysis?
Thanks a lot!
I got some illumina data which obviously has very small insert size as many of the read pairs have >95% overlap. I went back to illumina library protocol and feel that it generally suggests a library insert size longer than the total length of the paired-end read, that is, read pair does not overlap.
What is the optimal library insert size? Should I move forward for data analysis?
Thanks a lot!
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