Just wondering how much wgs library (prepared by Nextera xt kit) should be loaded on MiSeq using v3 600 cycle kit. We tried two times, 20 pM and 14 pM, both runs yielded only ~7 gb (half of 15 gb). We did several amplicon libraries which were pretty good. Are there any tricks? Thank you very much in advance.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Your experience indicates issues with correct quantification and loading. For good yield cluster density should be over 1200k/mm2 and this can be achieved with18-19 pM loading.
For specific diagnosis you can contact Tech support at Illumine or post more information about library (trace), quantification method, if bead normalisation was used and run summary page from SAV.
Latest Articles
Collapse
-
by seqadmin
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...-
Channel: Articles
05-24-2024, 01:16 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 06-03-2024, 06:55 AM
|
0 responses
12 views
0 likes
|
Last Post
by seqadmin
06-03-2024, 06:55 AM
|
||
Started by seqadmin, 05-30-2024, 03:16 PM
|
0 responses
27 views
0 likes
|
Last Post
by seqadmin
05-30-2024, 03:16 PM
|
||
Comprehensive Sequencing of Great Ape Sex Chromosomes Yields Insights into Evolution and Genetic Variability
by seqadmin
Started by seqadmin, 05-29-2024, 01:32 PM
|
0 responses
29 views
0 likes
|
Last Post
by seqadmin
05-29-2024, 01:32 PM
|
||
Started by seqadmin, 05-24-2024, 07:15 AM
|
0 responses
216 views
0 likes
|
Last Post
by seqadmin
05-24-2024, 07:15 AM
|
Comment