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  • #16
    Is a problem of focus alignment.
    You need to realign the Focus position.

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    • #17
      Was this ever resolved?
      I am encountering similar issues with focus errors.
      I know it's not my library because it was quantified by qPCR and we've sequenced this before.
      My flow cell stage still looks quite dirty even after cleaning it, but Illumina says it's fine.

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