Hello everyone,
It is my first time posting so I don't really know if I'm at the right place.
I am new to sequencing and still a student so please tell me if my question is a dumb question:
I'm working on a project in which we have sequencing datas of people coming from 2 platforms: one from Thermofischer and one from Illumina. However, the BAM file size per individual is 8 times bigger for the Thermofischer originated datas compared to the Illumina datas. How can that be explained ? Can it be the sequencing method (Sanger/ Ion torrent for Thermofischer), the coverage, or the alignment software used ? (For Illumina dataset, the iSAAC pipeline was used apparently).
I really want to get a better understanding of all these things so if you have advices or documents that might help me, I'll be happy to take it.
Thank you very much!!!
It is my first time posting so I don't really know if I'm at the right place.
I am new to sequencing and still a student so please tell me if my question is a dumb question:
I'm working on a project in which we have sequencing datas of people coming from 2 platforms: one from Thermofischer and one from Illumina. However, the BAM file size per individual is 8 times bigger for the Thermofischer originated datas compared to the Illumina datas. How can that be explained ? Can it be the sequencing method (Sanger/ Ion torrent for Thermofischer), the coverage, or the alignment software used ? (For Illumina dataset, the iSAAC pipeline was used apparently).
I really want to get a better understanding of all these things so if you have advices or documents that might help me, I'll be happy to take it.
Thank you very much!!!
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