Hello,
If I am sequencing a heterogeneous mixture of amplicons using a MiSeq (2x250bp) to ensure overlap, and there are some short amplicons (i.e. ~ 150 bp), what happens to the run? Will the rxns simply be wasted on the short amplicons, or will there be adverse affect on the data?
Thanks,
Andor
If I am sequencing a heterogeneous mixture of amplicons using a MiSeq (2x250bp) to ensure overlap, and there are some short amplicons (i.e. ~ 150 bp), what happens to the run? Will the rxns simply be wasted on the short amplicons, or will there be adverse affect on the data?
Thanks,
Andor
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