Does anyone have experience using ITS primers with the Illumina platforms (specifically MiSeq)?
I do not understand why the ITS primers require specific considerations on the Earth Microbiome website. http://www.earthmicrobiome.org/proto...standards/its/
Users at my university have used ITS Truseq fusion primers for quadruple indexing and sequenced successfully while ignoring this melting temperature issue. Does the addition of the itru and internal barcode segments render this issue null and void?
I do not understand why the ITS primers require specific considerations on the Earth Microbiome website. http://www.earthmicrobiome.org/proto...standards/its/
Users at my university have used ITS Truseq fusion primers for quadruple indexing and sequenced successfully while ignoring this melting temperature issue. Does the addition of the itru and internal barcode segments render this issue null and void?
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