Hi all. I am running some samples on the nextseq and I have seen that after cycle 5 the intensity drops dramatically. At cycle 1 intensity was alright but then it massively goes down. The cluster density is also very low although I have followed the protocol properly and diluted my library to 1.8 pM. Is there a way I can check on basespace if this is due to a faulty flow cell?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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