Hi! Can anyone tell me, why Illumina's sequencing systems have two lasers (or in NovaSeq, four lasers)? I am a bit confused how the system works. Do those lasers change constantly wavelengths to identify all the four bases, or do they stay in a specific wavelength? Thanks already in advance!
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The lasers excite at fixed wavelengths. The fluorophores emit at different respective wavelengths. The example given by kc3228 is for the dual color chemistries of the NextSeq and NovaSeq. The MiniSeq, Miseq, and Hiseq systems use a 4 color chemistry.
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Here's another doc from Illumina which also describes the CMOS flow cell and compares the various detection chemistries (1, 2, 4 color) used by Illumina.
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