Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • bacterial RNAseq using TruSeq Lib prep

    Hi all. I am in the process of starting RNAseq from bacterial samples and I am trying to identify the best strategy to do it. Given that bacterial RNA is not poly-adenylated, a random hexamer-based system is essential. Has anyone used the TruSeq stranded library prep kit for bacterial RNA? Or do you have a good kit to recommend? The samples will be run on the MiSeq or NextSeq systems. Any advice to guide me through the magical world of RNAseq will be much appreciated

  • #2
    We used the Truseq kit succesfully on Bacteria. 2 things are essential:
    - DNAse treatment of your RNA.
    - Good depletion efficiency for the ribosomal RNA. Ribozero kit are usually doing well.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Recent Developments in Metagenomics
      by seqadmin





      Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
      09-23-2024, 06:35 AM
    • seqadmin
      Understanding Genetic Influence on Infectious Disease
      by seqadmin




      During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

      Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
      09-09-2024, 10:59 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 10-02-2024, 04:51 AM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 10-01-2024, 07:10 AM
    0 responses
    21 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-30-2024, 08:33 AM
    0 responses
    25 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-26-2024, 12:57 PM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Working...
    X