Hello,
I used the Illumina Nextera Flex kit to sequence bacteria (WGS) and I got two warning that I usually do not get when using Nextera XT:
1. overrepresented sequences in the first read (R1) but not the second read (R2).
2. warning/error k-mer content
Why do I get such errors? Does this indicate sequencing bias?
I used the Illumina Nextera Flex kit to sequence bacteria (WGS) and I got two warning that I usually do not get when using Nextera XT:
1. overrepresented sequences in the first read (R1) but not the second read (R2).
2. warning/error k-mer content
Why do I get such errors? Does this indicate sequencing bias?
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