Hi all,
Just did some sequencing for a client and got a little odd plot using MultiQC for the "FastQ per base N-Content". It looks like the first 35 bp had problems with "N" then suddenly stopped. The percentage isn't high, but I don't understand what happened. Using trimmomatic removed these which appeared to be runs of "N". Anyone seen something like this before, or have an explanation for this?
Thanks,
-pete
Edit: We made these libraries using KAPA tagmentation kits, and there were no bad tiles (none) using FastQC.
Just did some sequencing for a client and got a little odd plot using MultiQC for the "FastQ per base N-Content". It looks like the first 35 bp had problems with "N" then suddenly stopped. The percentage isn't high, but I don't understand what happened. Using trimmomatic removed these which appeared to be runs of "N". Anyone seen something like this before, or have an explanation for this?
Thanks,
-pete
Edit: We made these libraries using KAPA tagmentation kits, and there were no bad tiles (none) using FastQC.
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