Hello!
I have 3 questions,can you help me please to understand?
1. Why almost only the pcr step in the library prep is cited as source of errors while bridge amplification not?
I have found only one paper that talks about the bridge pcr errors..why? I think that they are both pcr steps so they should have the same error rates,it is correct or not?
2. Why duplicates are creates only during pcr step in library prep and not durind brigde pcr? I haven't found nothing in literature about bridge pcr as source of duplicates..but why? They are both pcr steps so they should have the same probability to create duplicates..Is it false?
3. If I remove pcr duplicate, why I don't loose the massive paralles sequencing power of ngs? I know that the benefit of ngs that allow to reach high specificity in sequencing is to sequence a lot of time the same fragment..but if I remove duplicates from the analysis i don't loose this benefit?
Thank you in advance for your help!
I have 3 questions,can you help me please to understand?
1. Why almost only the pcr step in the library prep is cited as source of errors while bridge amplification not?
I have found only one paper that talks about the bridge pcr errors..why? I think that they are both pcr steps so they should have the same error rates,it is correct or not?
2. Why duplicates are creates only during pcr step in library prep and not durind brigde pcr? I haven't found nothing in literature about bridge pcr as source of duplicates..but why? They are both pcr steps so they should have the same probability to create duplicates..Is it false?
3. If I remove pcr duplicate, why I don't loose the massive paralles sequencing power of ngs? I know that the benefit of ngs that allow to reach high specificity in sequencing is to sequence a lot of time the same fragment..but if I remove duplicates from the analysis i don't loose this benefit?
Thank you in advance for your help!