I did several PCR reactions to test (had enough pre-pcr sample to spare). I tried 0.5, 1, 2, and 4 ul of my 10 nm working soln primer with both 15 and 18 cycles, and only using 4 ul got rid of the smears (which would be 8x more than I usually use for ChIP). Perhaps I had more sample than usual, or my primer soln was too dilute.
Can't answer your question about the ramp step but sounds like something worth trying.
Can't answer your question about the ramp step but sounds like something worth trying.
Comment