We are currently working through the TruSeq protocol to prep gDNA for hybridization to Nimblegen probes and PE sequencing on a GAIIx... Our problem is that we attempted using Invitrogen's eGels for size selection instead of the recommended gel excision (we have the reagents in-hosue). The bands on the gel were very weak and looked to be out of the size range. I spoke with someone at Illumina about the issue and they claimed to have the same problems when using egels. Has anyone had any success/experience with TruSeq and egels?
Also, we would prefer to use Qiagen cleanup instead of bead cleanup. Has anyone attempted this with TruSeq?
Thanks for any information!
BTS
Also, we would prefer to use Qiagen cleanup instead of bead cleanup. Has anyone attempted this with TruSeq?
Thanks for any information!
BTS
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