Hey All,
I have increased the length of index runs and decreased the length of the main reads. There is an interfance to say how long you want each read to go for. So in principle instead of 2x150 you could do one of the ends with 300 reads as there is enough reagents. However I assume the quality goes down.
I am using a miniSeq or HiSeq. I normally would use kits that are 2x150. However on one of my ends the primer had to be further away then I would like. Could I get Read1 to go 200 cycles and read2 to go 100 cycles? I know that I can program it in, but what problems may I encounter? Would the read quality fall and I end up with NNNN for the last 50 cycles? I know that this happens if you try and go 400 cycles on one end with MiSeq.
Thanks!
I have increased the length of index runs and decreased the length of the main reads. There is an interfance to say how long you want each read to go for. So in principle instead of 2x150 you could do one of the ends with 300 reads as there is enough reagents. However I assume the quality goes down.
I am using a miniSeq or HiSeq. I normally would use kits that are 2x150. However on one of my ends the primer had to be further away then I would like. Could I get Read1 to go 200 cycles and read2 to go 100 cycles? I know that I can program it in, but what problems may I encounter? Would the read quality fall and I end up with NNNN for the last 50 cycles? I know that this happens if you try and go 400 cycles on one end with MiSeq.
Thanks!
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