We have a very strange problem with the GA2X machine here. We spotted few bubbles while doing the leak check with the actual reagents after placing the flow cell. The bubbles were found in the inlet pipe pumping reagents into the Flow cell, within the flow cell and also in the syringes that pump the fluids out of the machine. We do not know how the buubles found their way into the pipes and syringes. We always make sure that the reagent bottles are kept filled all the time. An engineer had visited the lab recently and could not conclude on how the bubbles were introduced into the system and neither could he solve our bubble problem. Instead, he suggested us to start wth the run. After the run started, we found that in each lane, there were few tiles which were black, instead of being either blue, green or yelow. I could make out quite clearly that it was a bubble issue. Does anyone here wrking on a same kind of machine, ever had a similar problem? If ye, I would be very grateful to that person if he or she could suggest me a solution.
SANTOSH
SANTOSH
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