@Captainentropy
In the first 5 test cassettes I had it happen with 2 out of 5 wells.
But with the first new cassette it happened only in one well.
I am using 2% Ethidium Bromide free cassettes and I definitely did not touch the well walls or bottom for sure. Hope this improves and I just had bad luck with the first ones.
Have you ever run a PCR product on the Pippin Prep without a purification step like columns or beads in between?
Your adapter dilution sound very promising.
I think I will try it again. Thanks!!!
If you start out with 100ng you probably are also dependent on the high sensitivity chips. Have you ever had problems running samples from the Pippin Prep on an Agilent? The elution buffer contains much more salt than they recommend for these chips.
In the first 5 test cassettes I had it happen with 2 out of 5 wells.
But with the first new cassette it happened only in one well.
I am using 2% Ethidium Bromide free cassettes and I definitely did not touch the well walls or bottom for sure. Hope this improves and I just had bad luck with the first ones.
Have you ever run a PCR product on the Pippin Prep without a purification step like columns or beads in between?
Your adapter dilution sound very promising.
I think I will try it again. Thanks!!!
If you start out with 100ng you probably are also dependent on the high sensitivity chips. Have you ever had problems running samples from the Pippin Prep on an Agilent? The elution buffer contains much more salt than they recommend for these chips.
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