Hello,
I have just finished prepping multiplex samples for paired end Illumina sequencing using NEB products but after a bioanalyzer I have a lot of primer dimer at 75bp and around 120bp which seems even stronger than my bands at 300bp.
The sequencing facility does not want to sequence them due to the primer dimer problem.
Anybody else having the same problem?
I was suggested to re-gel purify all the samples but I am afraid that I will end up with not enough DNA to sequence.
Any other suggestion or the effect it could have if we sequence it like this?
Attached is a picture of the caliper data.
Thank you,
I have just finished prepping multiplex samples for paired end Illumina sequencing using NEB products but after a bioanalyzer I have a lot of primer dimer at 75bp and around 120bp which seems even stronger than my bands at 300bp.
The sequencing facility does not want to sequence them due to the primer dimer problem.
Anybody else having the same problem?
I was suggested to re-gel purify all the samples but I am afraid that I will end up with not enough DNA to sequence.
Any other suggestion or the effect it could have if we sequence it like this?
Attached is a picture of the caliper data.
Thank you,
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