Open question: How is the HiSeq/GAII at sequencing through triplet repeats? Can a long series of, say, CAGs cause a secondary DNA structure that causes the sequencer to have problems?
I ask because I've noticed bases in these regions having very low quality in some of my runs and wondered if others have noticed this.
I ask because I've noticed bases in these regions having very low quality in some of my runs and wondered if others have noticed this.